rune loss (Elden Ring)

I walked to Rennala through the academy with 40,000 runes, everything was waiting for a place to save, and the queen appeared so suddenly, and of course she killed me and the second time I didn’t get, but 40,000 runes is so sorry, damn it, I wanted to increase the level, I’m not in the mood to play anymore . And how many runes did you lose the most?